Determination of D-sorbitol in human erythrocytes by an enzymatic fluorometric method with an improved deproteinization procedure
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منابع مشابه
Determination of D-sorbitol in human erythrocytes by an improved enzymatic method with fluorometric detection.
In this enzymatic method to analyze erythrocytes for D-sorbitol, the erythrocytes were separated from plasma by centrifugation, washed with isotonic saline (9 g/L NaCl), then diluted threefold with more saline. We lysed 1.5 mL of the diluted erythrocytes with chloroform and precipitated the protein with 58 g/L HClO4 solution. The resulting supernates were mixed with buffer, NAD+, and sorbitol d...
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ژورنال
عنوان ژورنال: Annals of Clinical Biochemistry: International Journal of Laboratory Medicine
سال: 2001
ISSN: 0004-5632,1758-1001
DOI: 10.1258/0004563011900920